Highway 102 at Sistonen's Corners is now open after a transport truck obstructed the road on Sunday morning.
The highway was closed just before 8:30 a.m. when a tractor trailer unit jack knifed across the highway.
Ontario Provincial Police officers were redirecting motorists to Highway 11/17 and were able to open one lane around 9 a.m. Police reported both lanes had been opened at 11:10 a.m.
It was the second time the stretch of highway was closed in a 12-hour period.
Two tractor trailers collided Saturday night, prompting the OPP to close the highway between around 10:30 p.m. and 2 a.m. Sunday.